BGCexpress™ Dual Promoter Cloning Service
What used to take us 2 years just took 2 months, soup to nuts!! We will be moving to publish this now-deorphaned Natural Product straight away!
BGC Dual Promoter Vector Cloning
Get your BGC in a Streptomyces Inducible Expression Vector in 10 days!
- Dual-inducible promoters improve odds of turning on or over-expressing a pathway
- Up to 150 kb gene cluster clones from virtually any microbe
- Enables capture of hard-to-amplify sequences
- Delivered, verified clones. No library screening required
- IP protection for recombinant clone (and activities thereof)
- Customer retains all rights and ownership
Precision capture and cloning of intact pathways up to 150 kb
Send us your microbial sample & sequence and our scientists do the rest!
10 day turn around
Cheaper than other methods (e.g. TAR or traditional BAC cloning)
Multiple BAC vector options and any sequenced genome
Customer retains all rights and ownership
- Large secondary metabolism pathways, including repetitive polyketide and nonribosomal peptide natural products
- Genomic islands, virulence factors, proviruses, antibiotic biosynthetic pathways, etc
- Long operons, amplification resistant genes
What you provide
- Microbial cells
- Whole-genome sequence
- Biosynthetic gene cluster target of interest
What you receive
- Sequence-verified BAC clone of your region of interest
- Turn around time of 10 days!
- Customizable bacterial artificial chromosome (BAC) vector
505 S. Rosa Road
Madison, WI 53719
Varigen™ Biosciences, Natural Products by Design™ is an NIH- and NSF-funded effort to harness naturally occurring microbial diversity to develop the next generation of natural products through genomics and metagenomics. Varigen's team is led by researchers with over 125 years of combined experience.