DNAtrap™️ Biosynthetic Gene Cluster Cloning Service

DNAtrap™ technology provides custom cloning of biosynthetic gene clusters (BGCs) up to 100 kb in length. Varigen’s CRISPR/Cas9-based technology is significantly faster than competing techniques as we can deliver intact gene cluster clones from your sequenced microbe in 10 days.

Varigen has extensive experience cloning large, difficult DNA fragments, such as polyketide synthase (PKS), non-ribosomal peptide synthase (NRPS), and hybrid BGCs, as well as difficult to PCR fragments like virulence factor pathways. In these examples, unique restriction digest fragment patterns match the in silico simulated digest pattern, demonstrating the entire genomic fragment was correctly cloned into the BAC plasmid vector.

Advantages

• 100 kb gene cluster clones from virtually any microbe
• Enables capture of hard-to-amplify sequences
• Delivered, verified clones. No library screening required
• IP protection for recombinant clone (and activities thereof)
• Customer retains all rights and ownership

Exact
Precision capture and cloning of intact pathways up to 100 kb

Convenience
Send us your bacterial sample & sequence and our scientists do the rest!

Speed
10 day turn around

Cost
Cheaper than other methods (e.g. TAR or traditional BAC cloning)

Flexibility
Multiple BAC vector options and any sequenced genome

Confidentiality
Customer retains all rights and ownership

Example Targets

• Large secondary metabolism pathways, including repetitive polyketide and nonribosomal peptide natural products
• Genomic islands, virulence factors, proviruses, antibiotic biosynthetic pathways, etc
• Long operons, amplification resistant genes

What you provide

• Microbial cells
• Whole-genome sequence
• Biosynthetic gene cluster target of interest

What you receive

• Sequence-verified BAC clone of your region of interest
• Turn around time of 10 days!

Options

• Novel, inducible dual-promoter vector
• Customizable bacterial artificial chromosome (BAC) vector